Site-Specific N-glycosylation Analysis of Recombinant Proteins by LC/MS

Autor: Kevin, Canis, Estelle, Garénaux, Jean-François, Boe
Rok vydání: 2021
Předmět:
Zdroj: Methods in molecular biology (Clifton, N.J.). 2271
ISSN: 1940-6029
Popis: The glycosylation process is extremely heterogeneous, dynamic, and complex compared with any other post-translational modification of protein. In the context of recombinant glycoproteins, glycosylation is a critical attribute as glycans could dramatically alter protein functions and properties including activity, half-life, in vivo localization, stability, and, last but not least, immunogenicity. Liquid chromatography combined to mass spectrometry constitutes the most powerful analytical approach to achieve the comprehensive glycan profile description or comparison of glycoproteins. This chapter details a versatile yet straightforward LC-MS approach for sample preparation, analysis, and data interpretation, enabling the evaluation of site-specific N-glycosylation of recombinant glycoproteins.
Databáze: OpenAIRE