A novel glycosphingolipid expressed in pig kidney: Gal alpha 1-3Lewis(x) hexaglycosylceramide
| Autor: | D, Bouhours, J, Liaigre, J, Naulet, D, Maume, J F, Bouhours |
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| Rok vydání: | 1997 |
| Předmět: |
Magnetic Resonance Spectroscopy
Molecular Sequence Data Lewis X Antigen Oligosaccharides Enzyme-Linked Immunosorbent Assay Cross Reactions Spectrometry Mass Fast Atom Bombardment Ceramides Kidney Methylation Glycosphingolipids Epitopes Lewis Blood Group Antigens Carbohydrate Sequence Animals Humans Chromatography Thin Layer Chickens |
| Zdroj: | Glycoconjugate journal. 14(1) |
| ISSN: | 0282-0080 |
| Popis: | Immunodetection of thin layer chromatograms of neutral glycosphingolipids of pig kidney cortex with a polyclonal antibody directed against the Gal alpha 1-3Gal determinant revealed several glycosphingolipids reacting with different intensities. A minor glycosphingolipid was isolated by preparative high performance thin layer chromatography. It was characterized as a type 2 hexaglycosylceramide with the following structure Gal alpha 1-3Gal beta 1-4(Fuc alpha 1-3)GlcNAc beta 1-3Gal beta 1-4Glc beta 1-Cer by fast atom bombardment- and desorption-chemical ionization-mass spectrometry, methylation analysis and hydrolysis with alpha-galactosidase followed by immunostaining with an anti-Lewis(x) monoclonal antibody. The proton NMR spectrum was found compatible with the proposed structure. Two other glycosphingolipids carrying the new determinant were partially characterized as an octa- and a branched-dodecaglycosylceramide. The expression of the Gal alpha 1-3 Lewis(x) determinant appeared to be developmentally regulated as it increased with age. The characterization of Gal alpha 1-3Le(x) in pig kidney indicates a new epitope capable of recognition by human natural antibodies in the context of xenotransplantation of pig organs to man. It also adds new members to the family of Le(x)-based glycolipids. |
| Databáze: | OpenAIRE |
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