| Autor: |
Francisco, Capani, Ezequiel, Saraceno, Valeria Romina, Boti, Laura, Aon-Bertolino, Juan Carlos, Fernández, Fernándo, Gato, Maria Sol, Kruse, Maria Sol, Krause, Lisandro, Giraldez, Mark H, Ellisman, Héctor, Coirini |
| Rok vydání: |
2008 |
| Předmět: |
|
| Zdroj: |
Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al. 32(1) |
| ISSN: |
0327-9545 |
| Popis: |
Cellular and subcellular organization and distribution of actin filaments have been studied with various techniques. The use of fluorescence photo-oxidation combined with phalloidin conjugates with eosin has allowed the examination of the precise cellular and subcellular location of F-actin. Correlative fluorescence light microscopy and transmission electron microscopy studies of F-actin distribution are facilitated with this method for morphological and physiological studies. Because phalloidin-eosin is smaller than other markers, this method allows the analysis of the three-dimensional location of F-actin with high-resolution light microscopy, three-d serial sections reconstructions, and electron tomography. The combination of selective staining and three-dimensional reconstructions provide a valuable tool for revealing aspects of the synaptic morphology that are not available when conventional electron microscopy is used. By applying this selective staining technique and three-dimensional imaging, we uncovered the structural organization of actin in the postsynaptic densities in physiological and pathological conditions. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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