Use of a newly developed rapid microbial ATP bioluminescence assay to detect microbial contamination on poultry carcasses

Autor:	G R, Siragusa, W J, Dorsa, C N, Cutter, L J, Perino, M, Koohmaraie
Rok vydání:	1996
Předmět:	Microbiological Techniques Adenosine Triphosphate Biometry Time Factors Evaluation Studies as Topic Luminescent Measurements Colony Count Microbial Animals Poultry
Zdroj:	Journal of bioluminescence and chemiluminescence. 11(6)
ISSN:	0884-3996
Popis:	A newly developed rapid microbial ATP bioluminescence test (R-mATP) was shown to be an adequate means to assay the microbial load of poultry carcasses. This assay utilizes differential extraction and filtration to separate somatic from microbial ATP in a very rapid timeframe. The assay requires approximately 5 min to complete; approximately 3.5 min to sample and 90 s analytical time. Correlation coefficient (r) between aerobic colony counts and R-mATP test results (n=329) was 0.82. Post-test probabilities to correctly classify carcasses with different levels of microbial contamination were as high as 98% for samples ofor = 3.5 log aerobic CFU per ml. Given the rapidity of this assay, the R-mATP holds potential for monitoring the microbial load of carcasses at poultry-processing critical control points. Other potential applications of this new version of the microbial ATP bioluminescence test are discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::f6c387347e67cf88cd274fc56328fabb https://pubmed.ncbi.nlm.nih.gov/9343313 Zobrazit plný text záznamu Plný text