| Popis: |
Ten lipid fractions were detected in patients with psoriasis by the method of unidimensional chromatography in a thin layer of silica gel with two solvent systems designed for separating a lipid extract of high-density lipoproteins (HDLP). Four phospholipids fractions were identified as follows: three main ones, i.e. phosphatidylcholine, lisophosphatidylcholine and sphyngomielin, and one minor fraction, i.e. phosphatidylethanolamine. The HDLP lipid comprises free cholesterol and its esters. Three glyceride fractions were detected: mono-, di- and triglicerides and free fatty acids. The ratio of the individual phospholipid fractions, on the one hand, and of free to ester-bound cholesterol was found in HDLP by computer-aided densitometry of chromatograms and coincided with the routinely obtained data. The described method designed to evaluate the HDLP lipid composition can be handy in investigations of HDLP in patients with psoriasis. |
