635 nm LED irradiation may prevent endoplasmic reticulum stress in MC3T3-E1 cells
Autor: | Hyejoung, Cho, Ok-Su, Kim, Byunggook, Kim, Ying, Yang, Jianan, Song, Danyang, Liu, Young, Kim, Sangmi, Jeon, Okjoon, Kim |
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Rok vydání: | 2021 |
Předmět: |
Osteoblasts
Cell Survival NF-E2-Related Factor 2 Blotting Western Eukaryotic Initiation Factor-2 3T3 Cells Alkaline Phosphatase Endoplasmic Reticulum Stress Real-Time Polymerase Chain Reaction Activating Transcription Factor 4 Mice Osteogenesis Animals Lasers Semiconductor Reactive Oxygen Species Cells Cultured Signal Transduction |
Zdroj: | Journal of molecular histology. 53(1) |
ISSN: | 1567-2387 |
Popis: | Although endoplasmic reticulum (ER) stress is thought to be involved in various diseases such as cancer, metabolic, and inflammatory disorders, the relationship between ER stress and bone diseases, are remains unclear. Tunicamycin-treated MC3T3-E1 osteoblasts were used as the ER stress model in this study. 635 nm light-emitting diode irradiation (635 nm-IR) was carried out for 1 h before and after inducing ER stress. To investigate the effects of 635 nm-IR on ER stress-induced MC3T3-E1 osteoblasts and the underlying mechanism, western blot, reverse transcription polymerase chain reaction, alkaline phosphatase and Alizarin red staining, 2',7'-dichlorodyhydrofluorescein diacetate assay, Fluo-3AM and immunocytochemistry were performed. Pretreatment with 635 nm-IR effectively prevented intracellular reactive oxygen species production and alleviated ER stress through the pancreatic ER kinase (PERK)-eukaryotic initiation factor 2 (eIF2)-activating transcription factor 4 (ATF4)-nuclear factor-like 2 (Nrf2) signaling pathway. Hence, 635 nm-IR may serve a protective role in the treatment of ER stress-related bone diseases. |
Databáze: | OpenAIRE |
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