| Autor: |
C, Feurgard, D, Bayle, F, Guézingar, C, Sérougne, A, Mazur, C, Lutton, J, Aigueperse, P, Gourmelon, D, Mathé |
| Rok vydání: |
1998 |
| Předmět: |
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| Zdroj: |
Radiation research. 150(1) |
| ISSN: |
0033-7587 |
| Popis: |
Male Wistar rats weighing 250 g were exposed to 4 Gy of neutrons/gamma radiation (3.33 Gy of neutrons and 0.66 Gy of gamma rays). After whole-body irradiation, plasma cholesterol and phospholipid levels increased up to 62 and 37%, respectively, at day 4 and then returned to control values 12 days after irradiation. Plasma triglyceride concentrations decreased concomitantly with decreased food intake after irradiation but remained higher than in pair-fed control rats. Plasma lipoproteins were separated by ultracentrifugation on a density gradient (1.006-1.210 g/ml). Four days after irradiation, most of the cholesterol (62% compared to 31% in controls, P0.001) is transported by apolipoprotein E-rich high-density lipoproteins. At the same time, plasma levels of apolipoproteins B and E were increased by 28 and 65%, respectively, while those of apolipoproteins AI and AIV were reduced by 21 and 59%, respectively. While in the liver of irradiated rats the apolipoprotein B/E receptor number was not modified, the hydroxymethylglutaryl coenzyme A reductase activity was fivefold higher than in control pair-fed rats. Four days after irradiation, the susceptibility of lipoproteins to peroxidation, as measured by the formation of conjugated dienes in the presence of Cu2+, was markedly increased while plasma vitamin E levels were decreased, demonstrating that irradiation reduces antioxidant stores markedly. These results suggest that such modified lipoproteins could be involved in radiation-induced vascular damage. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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