[Effect of gamma irradiation on immobilized trypsin]

Autor: V V, Ryl'tsev, L G, Vlasov, T I, Samoĭlova, L P, Volkovinskaia, L N, Bondareva
Rok vydání: 1984
Předmět:
Zdroj: Prikladnaia biokhimiia i mikrobiologiia. 20(5)
ISSN: 0555-1099
Popis: The effect of ionizing radiation of 0.05-10 Mrad on trypsin immobilized on dialdehyde cellulose was being studied. After irradiation the activity of native trypsin decreases by 25%, as compared with the initial, while the activity of immobilized trypsin remains constant. Before immobilization cellulose undergoes special pretreatment that leads to a decrease in the initial contamination. Some samples of modified cellulose were contaminated by staphylococcus culture (200,000 microbes per 0.2 g) and then exposed to irradiation of 0.05-0.4 Mrad. A distinct correlation between the irradiation dose (0.05-0.4 Mrad) and contamination of the object was registered.
Databáze: OpenAIRE