| Autor: |
Qing-Feng, Zhang, Jing-Ying, Hu, Yue-Xin, Yang, Wei-Qing, Pan |
| Rok vydání: |
2014 |
| Předmět: |
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| Zdroj: |
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitologyparasitic diseases. 26(2) |
| ISSN: |
1000-7423 |
| Popis: |
To analyze the efficiency and specificity of MSP2 alleles genotyping for Plasmodium falciparum isolates by Nest-PCR and PCR-RFLP.MSP2 alleles from Plasmodium falciparum isolates of Yunnan and Hainan were genotyped by Nest-PCR and PCR-RFLP, respectively, and the efficiency and specificity of the two methods were analyzed.The conventional Nest-PCR method could detect 79.8% (166/208) alleles of MSP2, and 65.7% (65/99) for 3D7 family, but could not identify the type of any allele. While PCR-RFLP showed 25.3% higher genotyping efficiency than Nest-PCR. Moreover, this method could identify the allele types.PCR-RFLP genotyping technique is more efficient and specific than conventional Nest-PCR, and it is a convenient tool in the study on molecular epidemiology of malaria. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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