Wheatgrass-Derived Polysaccharide Has Antiinflammatory, Anti-Oxidative and Anti-Apoptotic Effects on LPS-Induced Hepatic Injury in Mice
| Autor: | Sarmila, Nepali, Hyeon-Hui, Ki, Ji-Hyun, Lee, Hoon-Yeon, Lee, Dae-Ki, Kim, Young-Mi, Lee |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Lipopolysaccharides
Male Caspase 3 Anti-Inflammatory Agents NF-kappa B Apoptosis MAP Kinase Kinase Kinases Antioxidants Mice Inbred C57BL Mice Liver Proto-Oncogene Proteins c-bcl-2 Polysaccharides Animals Cytokines Chemical and Drug Induced Liver Injury Mitogen-Activated Protein Kinases Phosphorylation Triticum bcl-2-Associated X Protein |
| Zdroj: | Phytotherapy research : PTR. 31(7) |
| ISSN: | 1099-1573 |
| Popis: | Hepatic injury occurs frequently during sepsis, and polysaccharides isolated from plants have been reported to have antiinflammatory and antioxidant effects in various models. However, the effect of wheatgrass-derived polysaccharide (WGP) has not been previously studied. In the present study, we investigated the effect of WGP on lipopolysaccharide (LPS)-induced hepatic injury in mice. Mice were pre-treated with WGP (100 or 200 mg/kg daily for 2 days) and then challenged with LPS (1 mg/kg, intraperitoneal), and sacrificed after 12 h. Wheatgrass-derived polysaccharide decreased serum aminotransferase levels and histological changes as compared with LPS-challenged mice. Wheatgrass-derived polysaccharide also significantly inhibited LPS-induced pro-inflammatory cytokine up-regulation and improved the oxidative status of liver tissues. Furthermore, these effects were found to be mediated by the suppression of the transcriptional activity of nuclear factor-kappa B (NF-κB), due to inhibitions of transforming growth factor beta (TGF-β)-activated kinase (TAK)-1 phosphorylation and inhibition of kappa B (IκB)-α degradation. In addition, WGP inhibited the activations of mitogen-activated protein kinases (MAPKs). Wheatgrass-derived polysaccharide also attenuated hepatic cell death by modulating caspase-3 and apoptosis associated mitochondrial proteins, such as, B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X (Bax). Taken together, WGP possesses antiinflammatory, anti-oxidant and anti-apoptotic activity and ameliorates LPS-induced liver injury in mice. Copyright © 2017 John WileySons, Ltd. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text