| Autor: |
Sarah, Kitson, Vanitha N, Sivalingam, James, Bolton, Rhona, McVey, Mashid, Nickkho-Amiry, Melanie E, Powell, Alexandra, Leary, Hans W, Nijman, Remi A, Nout, Tjalling, Bosse, Andrew G, Renehan, Henry C, Kitchener, Richard J, Edmondson, Emma J, Crosbie |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Modern Pathology |
| ISSN: |
1530-0285 |
| Popis: |
Ki-67, a marker of cellular proliferation, is increasingly being used in pre-surgical window studies in endometrial cancer as a primary outcome measure. Unlike in breast cancer, however, there are no guidelines standardizing its measurement and its clinical relevance as a response biomarker is undetermined. It is, therefore, imperative that Ki-67 scoring protocols are optimized and its association with patient survival rigorously evaluated, in order to be able to clinically interpret the results of these studies. Using the International Ki-67 in Breast Cancer Working Group guidelines as a basis, whole slide, hot spot and invasive edge scoring protocols were evaluated using endometrial biopsies and hysterectomy specimens from 179 women. Whole sections and tissue microarrays, manual and semi-automated scoring using Definiens Developer software were additionally compared. Ki-67 scores were related to clinicopathological variables and cancer-specific survival in uni- and multivariate analysis. Against criteria of time efficiency, intra- and inter-observer variability and consistency, semi-automated hot spot scoring was the preferred method. Ki-67 scores positively correlated with grade, stage and depth of myometrial invasion (P-values all |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink