Use of 33P for Sanger DNA sequencing
| Autor: | R J, Zagursky, P S, Conway, M A, Kashdan |
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| Rok vydání: | 1991 |
| Předmět: | |
| Zdroj: | BioTechniques. 11(1) |
| ISSN: | 0736-6205 |
| Popis: | The use of [alpha-33P]deoxyadenosine 5'-triphosphate ([alpha-33P]dATP) in DNA sequencing has been described. 33P has a maximum beta-emission energy that is 50% stronger than 35S, but fivefold weaker than 32P. As a result, sequences generated using [alpha-33P]dATP have short exposure times like 32P, yet they maintain band resolution similar to 35S. Handling of [alpha-33P]dATP is straightforward because no special lead or Plexiglas shielding is necessary. |
| Databáze: | OpenAIRE |
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