| Autor: |
K, Severinov, D, Fenyö, E, Severinova, A, Mustaev, B T, Chait, A, Goldfarb, S A, Darst |
| Rok vydání: |
1994 |
| Předmět: |
|
| Zdroj: |
The Journal of biological chemistry. 269(33) |
| ISSN: |
0021-9258 |
| Popis: |
Ribonucleotide analogs bound in the initiating site of Escherichia coli RNA polymerase holoenzyme in open promoter complexes were cross-linked to the beta and sigma 70 subunits. Using limited proteolysis and chemical degradation, the cross-link site in sigma 70 was mapped to a segment between amino acids Glu508 and Met561 containing the C-terminal part of conserved region 3. This result, when reconciled with genetic data on the interaction of sigma 70 conserved regions 2 and 4 with the -10 and -35 promoter regions, respectively, allows us to model the orientation of the sigma 70 subunit domains within the open promoter complex. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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