[The principles of creating standard serum panels with a low concentration of HIV-1 antibodies for the quality control of commercial immunoenzyme test systems]

Autor: A N, Kanev, E A, Gutova, G A, Lomanova, M S, Vorob'eva, E Iu, Shalaev, S N, Zagrebel'nyĭ
Rok vydání: 1993
Předmět:
Zdroj: Voprosy virusologii. 38(2)
ISSN: 0507-4088
Popis: The main approaches are defined to the production of sera with the required concentration of antibodies to HIV by the dilution method and to formation on their basis of serum panels according to theoretical distribution of optic density (OD) values. It was shown to be principally possible to prepare panels of positive sera with low concentration of HIV-specific antibodies in a dry stable form, and their practical importance in the evaluation of the sensitivity of enzyme immunoassays was demonstrated. The evaluation of the quality of commercial test systems is based on the position and shape of the histogram of OD values distribution for panel sera for the controlled test systems.
Databáze: OpenAIRE