| Autor: |
Chunling, Mo, Yueyue, Yang, Ning, Chen, Xiushan, Yang, Shen, Tian |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Sheng wu gong cheng xue bao = Chinese journal of biotechnology. 30(9) |
| ISSN: |
1000-3061 |
| Popis: |
In this study, we constructed a yeast consortium surface-display expression system by using Flo1 as an anchor protein. Endoglucanase II (EGII) and cellobiohydrolase II (CBHII) from Trichoderma reesei, and β3-glucosidase 1 (BGLI) from Aspergillus aculeatus were immobilized on Saccharomyces cerevisiae Y5. We constructed the cellulose-displaying expression yeast consortium (Y5/fEGII:Y5/fCBHII:Y5/fBGLI = 1:1:1) and investigated the enzymatic ability and ethanol fermentation. The displayed cellulolytic enzymes was stabile during the 96-h fermentation. The yeast consortium produced 0.77 g/L ethanol from 10 g/L phosphoric acid swollen cellulose (PASC) within 96 h. The yield (in grams of ethanol produced per gram of carbohydrate consumed) was 0.35 g/g, which correspond to 68.6% of the theoretical yield. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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