Structure and expression of the mouse gene encoding the endozepine-like peptide from haploid male germ cells
| Autor: | M, Valentin, M, Balvers, W, Pusch, G F, Weinbauer, J, Knudsen, R, Ivell |
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| Rok vydání: | 2000 |
| Předmět: |
Homeodomain Proteins
Male Base Sequence Transcription Genetic Molecular Sequence Data Ovary Fushi Tarazu Transcription Factors Proteins Receptors Cytoplasmic and Nuclear DNA Steroidogenic Factor 1 Immunohistochemistry Spermatozoa DNA-Binding Proteins Mice Nuclear Receptor Subfamily 6 Group A Member 1 Sequence Homology Nucleic Acid Testis Animals Female Cloning Molecular Peptides Promoter Regions Genetic Pseudogenes Transcription Factors |
| Zdroj: | European journal of biochemistry. 267(17) |
| ISSN: | 0014-2956 |
| Popis: | The endozepine-like peptide (ELP) represents a testis-specific isoform of the ubiquitous acyl-CoA binding protein (ACBP) and is highly expressed in late haploid stages of male germ cell development. The genomic sequence of the functional ELP gene as well as that of a pseudogene were analysed from independent bacteriophage clones of a 129sv mouse genomic library. Unlike the ACBP gene, which comprises four exons, the ELP gene has only a single intron within the region of the 5' untranslated region, suggesting that, like some other haploid expressed genes, the ELP gene might have evolved by retroposon-mediated gene duplication. Primer extension analysis was used to define the start site for transcription and hence the 5' promoter region. Electrophoretic mobility shift analysis was carried out on this region comparing nuclear extracts from adult mouse testis with those from mouse liver. Several testis-specific DNA-protein complexes were observed throughout 700 bp upstream of the transcription start site. One of these could be identified as corresponding to a steroidogenic factor-1 (SF-1) binding element. Further analysis using pure transcription factors showed that this element at position -340 was able to bind specifically to both SF-1 and to the germ cell nuclear factor (GCNF). Immunohistochemical analysis using an ELP-specific antibody showed that expression was very restricted within the testis to the postmeiotic germ cells, and in the ovary to interstitial/luteal cells, cell-types known to express GCNF and SF-1, respectively. Testes of CREM-tau knockout mice, lacking all spermatogenic stages later than round spermatids, were devoid of ELP immunoreactivity, whereas in RAD6 knockout mice the few remaining elongated spermatids were clearly defined by this excellent late haploid marker product. The ELP gene and its product thus offer an ideal system with which to investigate the differentiation of late haploid stages of spermatogenesis. |
| Databáze: | OpenAIRE |
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