| Autor: |
A T, Orawski, W H, Simmons |
| Rok vydání: |
1992 |
| Předmět: |
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| Zdroj: |
Agents and actions. Supplements. 38 |
| ISSN: |
0379-0363 |
| Popis: |
Aminopeptidase P that hydrolyzes the Arg1-Pro2-bond of bradykinin was solubilized from rat lung microsomes using phosphatidylinositol-specific phospholipase C. The enzyme was purified 420-fold by chromatography on decylagarose (two steps), omega-aminodecyl-agarose and DEAE-Sephacel. A single stained band was observed following native gradient (4-15%) polyacrylamide gel electrophoresis. Dipeptidylaminopeptidase IV-like activity was also present in the final preparation and co-migrated with aminopeptidase P in the above gel system. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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