Determination of a novel integron-located variant (blaOXA -320 ) of Class D β-lactamase in Proteus mirabilis

Autor:	Aysegul Copur, Cicek, Azer Ozad, Duzgun, Aysegul, Saral, Cemal, Sandalli
Rok vydání:	2013
Předmět:	Turkey Drug Resistance Multiple Bacterial RNA Ribosomal 16S Molecular Sequence Data Humans Sequence Analysis DNA Urine Proteus mirabilis beta-Lactamases Integrons
Zdroj:	Journal of basic microbiology. 54(10)
ISSN:	1521-4028
Popis:	Proteus mirabilis (P. mirabilis) is one of Gram-negative pathogens encountered in clinical specimens. A clinical isolate (TRP41) of P. mirabilis was isolated from a Turkish patient in Turkey. The isolate was identified using the API 32GN system and 16S rRNA gene sequencing and it was found resistant to ampicillin/sulbactam, piperacillin, tetracycline, and trimethoprim/sulfamethoxazole. This isolate was harboring a Class 1 integron gene cassette and its DNA sequence analysis revealed a novel blaOXA variant exhibiting one amino acid substitution (Asn266Ile) from blaOXA-1 . This new variant of OXA was located on Class 1 integron together with aadA1 gene encoding aminoglycoside-modifying enzymes. According to sequence records, the new variant was named as blaOXA-320 . Cassette array and size of integron were found as blaOXA-320 -aadA1 and 2086 bp, respectively. The blaOXA-320 gene is not transferable according to conjugation experiment. In this study, we report the first identification of blaOXA-320 -aadA1 gene cassette, a novel variant of Class D β-lactamase, in P. mirabilis from Turkey.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::f48a17376f0d39036e0e2414cdc372e3 https://pubmed.ncbi.nlm.nih.gov/24027220 Zobrazit plný text záznamu Plný text