| Autor: |
Hoon Seok, Yoon, Hee-Chul, Ko, Sang Suk, Kim, Kyung Jin, Park, Hyun Joo, An, Young Hun, Choi, Se-Jae, Kim, Nam-Ho, Lee, Chang-Gu, Hyun |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Natural product communications. 10(3) |
| ISSN: |
1934-578X |
| Popis: |
In order to test the effectiveness of tangeretin at ameliorating melanoma and melanoma-associated depigmentation, western blotting was used to assess the melanin content of treated melanoma cells. Tangeretin, a 4',5,6,7,8-pentamethoxyflavone, was found to trigger intracellular melanin production in a concentration-dependent manner in B16/F10 murine melanoma cells. Melanin content increased 1.74-fold in response to treatment with 25 μM of tangeretin, compared to that in non-treated cells. Examination of melanogenic protein expression showed that tyrosinase, tyrosinase-related protein (TRP)-1, and extracellular signal-regulated kinase (ERK) 1/2 levels increased in a dose-dependent manner. Furthermore, the expression of cyclic adenosine monophosphate response element binding protein (CREB) and microphthalmia transcription factor (MITF) was increased by tangeretin in 1 h and 4 h, respectively. Tangeretin- upregulated melanogenesis was suppressed by ERK 1/2 inhibitor and not by ERK1 inhibitor. These results suggest that tangeretin has therapeutic potential for melanoma and melanoma-associated depigmentation because it can induce hyperpigmentation through the activation of melanogenic signaling proteins and initiation of sustained ERK2 expression. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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