| Autor: |
You-Zhi, Li, Zhi-Yao, Chen, Hui, Wang, Huan, Huang, Qin-Xin, Song, Guo-Hua, Zhou |
| Rok vydání: |
2012 |
| Předmět: |
|
| Zdroj: |
Zhonghua nan ke xue = National journal of andrology. 18(2) |
| ISSN: |
1009-3591 |
| Popis: |
To establish a high-sensitivity, high-specificity and low-cost hydrogel chip platform for the clinical screening of Y chromosome microdeletions.Site-specific extended primers with a common sequence at the 5' end were used for hybridizing with the target. The Cy5-dUTP was incorporated into the products by primer extension, and the products were labeled with fluorescence. Then the extended products were added to the chip for hybridizing with acrylamide-modified common probes immobilized on the chip. After removal of the free Cy5-dUTP by electrophoresis, the signals were obtained by fluorescence scanning. And the detecting conditions of this method were optimized.SY254 of 9 samples was successfully detected with the hydrogel chip. The results showed that 3 were normal and the other 6 with microdeletions (1 female sample as a negative control), which coincided with the results of conventional multiplex PCR-electrophoresis.The hydrogel chip platform we established has provided a new technique for the detection of Y chromosome microdeletions, and is beneficial to the diagnosis and treatment of male infertility. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
