TGF-beta antisense oligonucleotides modulate expression of matrix metalloproteinases in isolated fibroblasts from radiated skin
| Autor: | Katrin, Riedel, T, Kremer, H, Ryssel, F, Riedel, U R, Goessler, E, Koellensperger, G, Germann, M, Sauerbier |
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| Rok vydání: | 2008 |
| Předmět: |
Wound Healing
Gene Expression Fibroblasts Oligonucleotides Antisense Immunoenzyme Techniques Matrix Metalloproteinase 9 Transforming Growth Factor beta Humans Matrix Metalloproteinase 2 RNA Messenger Fluorescent Antibody Technique Indirect Radiation Injuries Biomarkers Cells Cultured Oligonucleotide Array Sequence Analysis Skin |
| Zdroj: | In vivo (Athens, Greece). 22(1) |
| ISSN: | 0258-851X |
| Popis: | Transforming growth factor-beta (TGF-beta) has been identified as an important component of wound healing. Recent developments in molecular therapy offer exciting prospects for the modulation of wound healing, specifically those targeting TGF-beta. The purpose of this study was to analyze the effect of TGF-beta targeting on the expression of matrix metalloproteinases (MMPs) in fibroblasts isolated from radiation-induced chronic dermal wounds.The expression of MMPs in tissue samples from radiation-induced chronic dermal wounds was investigated by immunohistochemistry and microarray technique. The effect of TGF-beta targeting using antisense oligonucleotides on the expression of MMPs in isolated fibroblasts was analysed by ELISA and multiplex RT-PCR.Immunohistochemical investigation and microarray analysis demonstrated an increased expression of MMP protein and mRNA in tissue samples from radiation-induced chronic dermal wounds compared to normal human skin. Antisense TGF-beta oligonucleotide treatment significantly down-regulated MMP secretion in vitro.TGF-beta antisense oligonucleotide technology may be a potential therapeutic option for the inhibition of proteolytic tissue destruction in radiation-induced chronic wounds. |
| Databáze: | OpenAIRE |
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