Transgenic embryo yield is increased by a simple, inexpensive micropipet treatment

Autor:	K, Gundersen, T A, Hanley, J P, Merlie
Rok vydání:	1993
Předmět:	Mice Genetic Techniques Microinjections Animals Mice Transgenic DNA Embryo Mammalian
Zdroj:	BioTechniques. 14(3)
ISSN:	0736-6205
Popis:	A method for the treatment of micropipet tips for injection of DNA into pronuclei of mouse embryos is described. The method requires only standard laboratory equipment, a magnetic stirrer and an inexpensive commercially available grinding powder. The method involves little extra time and no special skills. After introduction of the method, the time required for injections was reduced by about 30%, and the yield of surviving embryos was increased by 28%.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::f2a712629c6c8f20f6d5fba166320e3b https://pubmed.ncbi.nlm.nih.gov/8457346 Zobrazit plný text záznamu