| Autor: |
M E, Rodie, M A V, Mudaliar, P, Herzyk, M, McMillan, M, Boroujerdi, S, Chudleigh, E S, Tobias, S F, Ahmed |
| Rok vydání: |
2017 |
| Předmět: |
|
| Zdroj: |
European journal of endocrinology. 177(4) |
| ISSN: |
1479-683X |
| Popis: |
It is unclear whether a short-term change in circulating androgens is associated with changes in the transcriptome of the peripheral blood mononuclear cells (PBMC).To explore the effect of hCG stimulation on the PBMC transcriptome, 12 boys with a median age (range) of 0.7 years (0.3, 11.2) who received intramuscular hCG 1500u on 3 consecutive days as part of their investigations underwent transcriptomic array analysis on RNA extracted from peripheral blood mononuclear cells before and after hCG stimulation.Median pre- and post-hCG testosterone for the overall group was 0.7 nmol/L (0.5, 6) and 7.9 nmol/L (0.5, 31.5), respectively. Of the 12 boys, 3 (25%) did not respond to hCG stimulation with a pre and post median serum testosterone of0.5 nmol/L and0.5 nmol/L, respectively. When corrected for gene expression changes in the non-responders to exclude hCG effects, all 9 of the hCG responders consistently demonstrated a 20% or greater increase in the expression ofThe identification of a dynamic and androgen-responsive PBMC transcriptome extends the potential value of the hCG test for the assessment of androgen sufficiency. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
