| Autor: |
J A, Herrera, G, Calderon, C, Cruz, M A, Avila, G E, Quintero, R C, Fierro |
| Rok vydání: |
2018 |
| Předmět: |
|
| Zdroj: |
Cryo letters. 38(4) |
| ISSN: |
0143-2044 |
| Popis: |
That cryopreservation can induce alterations in sperm.The goal of this study was to evaluate sperm quality and distribution of N-acetylglucosamine, sialic acid and mannose residues in sperm cryopreserved of red-tailed hawk (Buteo jamaicensis).We studied twenty samples of ejaculated semen for each cryoprotectant dimethylsulfoxide or polyvinylpyrrolidone. Carbohydrate identification was carried out with lectins Triticum vulgaris agglutinin to N-acetylglucosamine and sialic acid and Concanavalia ensiformis for mannose residues. Sperm viability was not altered but motility decreased significantly with both crioprotectants compared with fresh sperm.Neither the number of WGA positive sperm nor the distribution of N-acetylglucosamine and/or sialic acid residues were affected by the cryopreservation procedure. The sperm proportion with fluorescence associated with the presence of mannose residues was higher in thawed sperm.Values obtained with the cryopreservation technique proposed in this study by freezing drops in liquid nitrogen, were within normal parameters established for good quality fresh semen. We can say that it can be used for assisted reproduction of Buteo jamaicensis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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