| Autor: |
Nathanael, Miller, Dane, Wolf, Nour, Alsabeeh, Kiana, Mahdaviani, Mayuko, Segawa, Marc, Liesa, Orian S, Shirihai |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 2276 |
| ISSN: |
1940-6029 |
| Popis: |
Changes to mitochondrial architecture are associated with various adaptive and pathogenic processes. However, quantification of changes to mitochondrial structures is limited by the yet unmet challenge of defining the borders of each individual mitochondrion within an image. Here, we describe a novel method for segmenting primary brown adipocyte (BA) mitochondria images. We describe a granular approach to quantifying subcellular structures, particularly mitochondria in close proximity to lipid droplets: peridroplet mitochondria. In addition, we lay out a novel machine-learning-based mitochondrial segmentation method that eliminates the bias of manual mitochondrial segmentation and improves object recognition compared to conventional thresholding analyses. By applying these methods, we discovered a significant difference between cytosolic and peridroplet BA mitochondrial H |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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