Novel Long Noncoding RNA, Macrophage Inflammation-Suppressing Transcript (
| Autor: | Kenneth, Stapleton, Sadhan, Das, Marpadga A, Reddy, Amy, Leung, Vishnu, Amaram, Linda, Lanting, Zhuo, Chen, Lingxiao, Zhang, Rengasamy, Palanivel, Jeffrey A, Deiuliis, Rama, Natarajan |
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| Rok vydání: | 2020 |
| Předmět: |
Male
obesity Poly (ADP-Ribose) Polymerase-1 Down-Regulation macrophage Cell Line Poly ADP Ribosylation lncRNA Animals Humans Inflammation Metabolic Syndrome Basic Sciences Cholesterol LDL Macrophage Activation Lipid Metabolism Chromatin Up-Regulation adipose tissue Mice Inbred C57BL ComputingMethodologies_DOCUMENTANDTEXTPROCESSING Cytokines RNA Long Noncoding transcriptome |
| Zdroj: | Arteriosclerosis, Thrombosis, and Vascular Biology |
| ISSN: | 1524-4636 |
| Popis: | Supplemental Digital Content is available in the text. Objective: Systemic low-grade inflammation associated with obesity and metabolic syndrome is a strong risk factor for the development of diabetes mellitus and associated cardiovascular complications. This inflammatory state is caused by release of proinflammatory cytokines by macrophages, especially in adipose tissue. Long noncoding RNAs regulate macrophage activation and inflammatory gene networks, but their role in macrophage dysfunction during diet-induced obesity has been largely unexplored. Approach and Results: We sequenced total RNA from peritoneal macrophages isolated from mice fed either high-fat diet or standard diet and performed de novo transcriptome assembly to identify novel differentially expressed mRNAs and long noncoding RNAs. A top candidate long noncoding RNA, macrophage inflammation-suppressing transcript (Mist), was downregulated in both peritoneal macrophages and adipose tissue macrophages from high-fat diet–fed mice. GapmeR-mediated Mist knockdown in vitro and in vivo upregulated expression of genes associated with immune response and inflammation and increased modified LDL (low-density lipoprotein) uptake in macrophages. Conversely, Mist overexpression decreased basal and LPS (lipopolysaccharide)-induced expression of inflammatory response genes and decreased modified LDL uptake. RNA-pull down coupled with mass spectrometry showed that Mist interacts with PARP1 (poly [ADP]-ribose polymerase-1). Disruption of this RNA-protein interaction increased PARP1 recruitment and chromatin PARylation at promoters of inflammatory genes, resulting in increased gene expression. Furthermore, human orthologous MIST was also downregulated by proinflammatory stimuli, and its expression in human adipose tissue macrophages inversely correlated with obesity and insulin resistance. Conclusions: Mist is a novel protective long noncoding RNA, and its loss during obesity contributes to metabolic dysfunction and proinflammatory phenotype of macrophages via epigenetic mechanisms. |
| Databáze: | OpenAIRE |
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