| Autor: |
M V, Kulak, N A, Netesova, P A, Belavin, E V, Seregina, G M, Ignat'ev |
| Rok vydání: |
2008 |
| Předmět: |
|
| Zdroj: |
Molekuliarnaia genetika, mikrobiologiia i virusologiia. (1) |
| ISSN: |
0208-0613 |
| Popis: |
Recombinant proteins rN (nucleocapsid) and rH/Nh (hemagglutinin) of the measles virus strain NovO/96 of genotype A were obtained. The immunobiological properties of the proteins were studied in the reaction with a panel of positive and negative sera. Mice of the line Balb/C were immunized with recombinant proteins and native antigen of the measles virus strain NovO/96 in order to obtain hyperimmune serum and its analysis using ELISA (enzyme-linked immunosorbent assay) and PRN (plaque reduction neutralization). The hyperimmune serum against recombinant proteins and native antigen of the measles virus strain NovO/96 were found to be highly active in ELISA. The antibodies against the proteins rN and rH/Nh were found to be able to neutralize the virus in titer 1:13.5 and 1:22.9, respectively. The neutralization titer of the antibodies generated against native antigen of the measles virus strain NovO/96 was 1:25.7. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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