| Autor: |
M M, Hossain, H, Tsuchie, M A, Detorio, H, Shirono, C, Hara, A, Nishimoto, A, Saji, J, Koga, N, Takata, J K, Maniar, D G, Saple, K, Taniguchi, S, Kageyama, H, Ichimura, T, Kurimura |
| Rok vydání: |
1998 |
| Předmět: |
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| Zdroj: |
Acta virologica. 42(1) |
| ISSN: |
0001-723X |
| Popis: |
A search for gene(s) associated with anti-human immunodeficiency virus type 1 (HIV-1) activity of CD8+ T cells was attempted using molecular cloning and the relation between the anti-HIV activity of CD8+ T cells and the interleukin-9 receptor alpha chain (IL-9R-alpha) mRNA expression from the cDNA clones obtained was examined. The anti-HIV-1 activity of CD8+ T cell culture supernatants was assessed by measuring the level of HIV-1 replication of a CD4+ T cell line transfected with an infectious HIV-1 DNA clone. IL-9R-alpha mRNA was assayed by reverse transcriptase-polymerase chain reaction (RT-PCR). Of 5 cases showing high level of anti-HIV-1 activity (more than 80% suppression of HIV-1 replication), the mRNA was detected in 4 cases. Of 10 cases showing low level of anti-HIV-1 activity (less than 80% suppression of HIV-1 replication), the mRNA was detected in one case. Soluble recombinant human IL-9 receptor (rhIL-9sR) did not suppress HIV-1 replication at a concentration of 1 microgram/ml. These data suggest that the IL-9R-alpha mRNA formation in CD8+ T cells may correlate with and play some role in the anti-HIV-1 activity of CD8+ T cells from HIV-1-infected individuals. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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