Interaction between Smad 3 and Dishevelled in murine embryonic craniofacial mesenchymal cells
| Autor: | D R, Warner, R M, Greene, M M, Pisano |
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| Rok vydání: | 2005 |
| Předmět: |
Mice
Inbred ICR Palate Dishevelled Proteins Embryonic Development Proteins Protein-Tyrosine Kinases Embryo Mammalian Phosphoproteins Culture Media DNA-Binding Proteins Mesoderm Wnt Proteins Mice Transforming Growth Factor beta Maxilla Trans-Activators Animals Intercellular Signaling Peptides and Proteins Smad3 Protein Cells Cultured Adaptor Proteins Signal Transducing Glutathione Transferase Protein Binding Signal Transduction |
| Zdroj: | Orthodonticscraniofacial research. 8(2) |
| ISSN: | 1601-6335 |
| Popis: | To determine the in vivo interaction between Smad 3 and Dishevelled-1.Cell culture transfection followed by immunoprecipitation with specific antibodies.The Department of Molecular, Cellular, and Craniofacial Biology, Birth Defects Center, University of Louisville.Overexpression of myc-Smad 3.Western blotting of anti-Dishevelled immunoprecipitates for Smad 3.Smad 3 and Dishevelled isoforms-1, -2, and -3 all bind Smad 3 in glutathione-S-transferase (GST) pull-down assays and Smad 3 binds to Dishevelled-1 in vivo. Stimulation of the transforming growth factor beta (TGFbeta) pathway leads to increased binding of Smad 3 and Dishevelled-1 in vivo.Smad 3 binds all three known isoforms of Dishevelled and binds Dishevelled 1 in vivo. TGFbeta signaling modulates the interaction between Smad 3 and Dishevelled-1. |
| Databáze: | OpenAIRE |
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