| Autor: |
Pei, Yan, Da-Yan, Liang, Wen-Hao, Xu, Lu, Xue, Meng-Fei, Yu, Jin-Hua, Shen, Qing-Hua, Liu, Yong-Bo, Peng |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Sheng li xue bao : [Acta physiologica Sinica]. 73(3) |
| ISSN: |
0371-0874 |
| Popis: |
S100 calcium binding protein A9 (S100A9) is involved in a variety of biological processes such as inflammation and tumor cell migration and invasion regulation. The purpose of this study was to construct S100A9 gene-edited mice by using CRISPR/Cas9 technology, thereby providing an animal model for exploring the biological functions of this gene. According to the S100A9 gene sequence, the single-stranded small guide RNA (sgRNA) targeting exons 2 and 3 was transcribed in vitro, and a mixture of Cas9 mRNA and candidate sgRNA was injected into mouse fertilized eggs by microinjection. Early embryos were obtained and transferred to surrogate mice, and F |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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