| Autor: |
De-biao, Chi, Lin, Li, Li-sha, Sun, Wen-feng, Ma |
| Rok vydání: |
2007 |
| Předmět: |
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| Zdroj: |
Nan fang yi ke da xue xue bao = Journal of Southern Medical University. 27(7) |
| ISSN: |
1673-4254 |
| Popis: |
To investigate the cell biological mechanism of koumine-induced apoptosis of human colonic adenocarcinoma cells.The effects of LoVo cell koumine on the membrane potential, mitochondrial membrane potential, concentration of cytosolic free calcium, reactive oxygen species (ROS) and gap junctional intercellular communication was observed by laser scanning confocal microscopy.Koumine lowered the membrane potential and mitochondrial membrane potential of LoVo cells and decreased the concentration of free cytosolic calcium, which was increased to a level higher than the basal one after addition of EDTA. Koumine also increased the reactive oxygen species and enhanced the gap junctional intercellular communication of LoVo cells. These findings may explain the possible mechanisms of koumine-induced LoVo cell apoptosis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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