[Cloning, expression and subcellular localization of a novel human gene-RNF122]
| Autor: | Li, Wang, Tai-ping, Shi, Lan, Wang, Chuan-fei, Yu, Ling-e, Zeng, Jing, Wang, Lu, Wang |
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| Rok vydání: | 2006 |
| Předmět: |
DNA
Complementary Microscopy Confocal Base Sequence Reverse Transcriptase Polymerase Chain Reaction Myocardium Recombinant Fusion Proteins Green Fluorescent Proteins Molecular Sequence Data Gene Expression Golgi Apparatus Membrane Proteins HL-60 Cells Zinc Fingers Sequence Analysis DNA Blotting Northern Endoplasmic Reticulum Cell Line Cell Line Tumor Humans Amino Acid Sequence Cloning Molecular Muscle Skeletal Lung |
| Zdroj: | Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences. 38(3) |
| ISSN: | 1671-167X |
| Popis: | To clone a functionally unknown gene RNF122 and analyze its expression pattern and subcellular localization.PCR was used to clone the novel gene-RNF122 from the mixed human tissue cDNA library. Bioinformatics analysis was used to identify structure characteristics of the gene. Northern blot was used to analyze its expression in normal tissues; RT-PCR was employed for its expression in cell lines and tumor tissues. By co-focal microscope, we identified its subcellular localization with organelle markers.A novel human gene-RNF122 was cloned, which was proved to have been expressed in several normal and tumor tissues and many cell lines, and localized in ER and Golgi apparatus.RNF122 is a novel gene which encodes a protein that has a classic RING domain. It is widely expressed in several tissues and cell lines. The encoded protein is localized in ER and Golgi apparatus, which indicates that it may play a role in the process of protein degradation. |
| Databáze: | OpenAIRE |
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