Malignant transformation of mammalian cells initiated by constitutive expression of the polo-like kinase
| Autor: | M R, Smith, M L, Wilson, R, Hamanaka, D, Chase, H, Kung, D L, Longo, D K, Ferris |
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| Rok vydání: | 1997 |
| Předmět: |
Microinjections
Cell Cycle Gene Expression Mice Nude Mitosis Cell Cycle Proteins 3T3 Cells DNA Neoplasms Experimental Saccharomyces cerevisiae Protein Serine-Threonine Kinases Transfection Mice Cell Transformation Neoplastic Proto-Oncogene Proteins Animals Humans Drosophila RNA Messenger Protein Kinases Cell Division |
| Zdroj: | Biochemical and biophysical research communications. 234(2) |
| ISSN: | 0006-291X |
| Popis: | Polo-like kinase (Plk) is the mammalian homologue of the Drosophila polo and Saccharomyces cerevisiae CDC5 genes, which are thought to be involved in regulating chromosomal segregation. Previously, we showed that transient ectopic expression of Plk could induce DNA synthesis in quiescent NIH 3T3 cells, suggesting that Plk might also have a function during G1 or S phase. Here we report that microinjection of Plk mRNA is sufficient to drive quiescent cells into mitosis and that constitutive expression of Plk in NIH 3T3 cells causes oncogenic focus formation. These transformed cells grow in soft agar and form tumors in nude mice. Because Plk expression has been shown to be high in various human tumors, we suggest that Plk may contribute to the promotion and/or progression of human cancers. |
| Databáze: | OpenAIRE |
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