Chromatographic methods for simultaneous determination of diiodohydroxyquinoline and metronidazole in their binary mixture
| Autor: | Nouruddin Wageih, Ali, Mohammed, Gamal, Mohammed, Abdelkawy |
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| Rok vydání: | 2013 |
| Předmět: |
Chromatography
Chromatography Reverse-Phase Chemistry Pharmaceutical Reproducibility of Results Buffers Hydrogen-Ion Concentration Reference Standards Metronidazole Calibration Iodoquinol Solvents Technology Pharmaceutical Spectrophotometry Ultraviolet Chromatography Thin Layer Chromatography High Pressure Liquid |
| Zdroj: | Pakistan journal of pharmaceutical sciences. 26(5) |
| ISSN: | 1011-601X |
| Popis: | Two chromatographic methods were developed for analysis ofdiiodohydroxyquinoline (DIHQ) and metronidazole (MTN). In the first method, diiodohydroxyquinoline and metronidazole were separated on TLC silica gel 60F254 plate using chloroform: acetone: glacial acetic acid (7.5: 2.5: 0.1, by volume) as mobile phase. The obtained bands were then scanned at 254 nm. The second method is a RP-HPLC method in which diiodohydroxyquinoline and metronidazole were separated on a reversed-phase C18 column using water : methanol (60 :40, V/V, PH=3.6 )as mobile phase at a flow rate of 0.7 mL.min-1 and UV detection at 220 nm. The mentioned methods were successfully used for determination of diiodohydroxyquinoline and metronidazole in pure form and in their pharmaceutical formulation. |
| Databáze: | OpenAIRE |
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