The Bisindole Alkaloid Caulerpin, from Seaweeds of the Genus
| Autor: | Alessandra M M, Lucena, Cássio R M, Souza, Jéssica T, Jales, Paulo M M, Guedes, George E C, de Miranda, Adolpho M A, de Moura, João X, Araújo-Júnior, George J, Nascimento, Kátia C, Scortecci, Barbara V O, Santos, Janeusa T, Souto |
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| Rok vydání: | 2018 |
| Předmět: |
Male
Indoles Colon Anti-Inflammatory Agents Drug Evaluation Preclinical Peritonitis Article caulerpin Mice Alkaloids Animals Caulerpa Humans Intestinal Mucosa anti-inflammatory activity ulcerative colitis Dose-Response Relationship Drug Dextran Sulfate tissue damage Zymosan Seaweed cytokines Mice Inbred C57BL Disease Models Animal Treatment Outcome Colitis Ulcerative |
| Zdroj: | Marine Drugs |
| ISSN: | 1660-3397 |
| Popis: | Caulerpin (CLP), an alkaloid from algae of the genus Caulerpa, has shown anti-inflammatory activity. Therefore, this study aimed to analyze the effect of CLP in the murine model of peritonitis and ulcerative colitis. Firstly, the mice were submitted to peritonitis to evaluate which dose of CLP (40, 4, or 0.4 mg/kg) could decrease the inflammatory infiltration in the peritoneum. The most effective doses were 40 and 4 mg/kg. Then, C57BL/6 mice were submitted to colitis development with 3% dextran sulfate sodium (DSS) and treated with CLP at doses of 40 and 4 mg/kg. The disease development was analyzed through the disease activity index (DAI); furthermore, colonic tissue samples were submitted to histological analysis, NFκB determination, and in vitro culture for cytokines assay. Therefore, CLP at 4 mg/kg presented the best results, triggering improvement of DAI and attenuating the colon shortening and damage. This dose was able to reduce the TNF-α, IFN-γ, IL-6, IL-17, and NFκB p65 levels, and increased the levels of IL-10 in the colon tissue. Thus, CLP mice treatment at a dose of 4 mg/kg showed promising results in ameliorating the damage observed in the ulcerative colitis. |
| Databáze: | OpenAIRE |
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