Overexpression of cyclin D1 is rare in human prostate carcinoma

Autor:	L M, Gumbiner, P H, Gumerlock, P C, Mack, S G, Chi, R W, deVere White, J L, Mohler, T G, Pretlow, J V, Tricoli
Rok vydání:	1999
Předmět:	Gene Expression Regulation Neoplastic Male Blotting Southern Reverse Transcriptase Polymerase Chain Reaction Blotting Western Prostatic Hyperplasia Tumor Cells Cultured Humans Prostatic Neoplasms Cyclin D1 DNA Neoplasm RNA Neoplasm DNA Primers
Zdroj:	The Prostate. 38(1)
ISSN:	0270-4137
Popis:	Overexpression of cyclin D1 has been documented in a number of human cancers. Increased expression of cyclin D1 can contribute to cellular transformation and abnormal proliferation.Quantitative RT-PCR and/or Western blot analysis were used to determine the level of cyclin D1 expression in 96 human prostate tumors, 15 benign prostate hyperplasias, 4 prostate cancer cell lines, and 3 xenografts.Our results demonstrate that 4.2% of the prostate tumors examined overexpressed cyclin D1 transcripts. In the cell lines, expression was normal, with the exception that reduced levels of cyclin D1 transcript and protein were observed in the DU145 cell line, as expected from cells with mutant RB. Normal levels of cyclin D1 were found in all xenograft tumors and BPH specimens examined.These data show that overexpression of cyclin D1 occurs rarely in human prostate tumors. However, when overexpression of cyclin D1 does occur, it may identify a subset of tumors with a different molecular biology.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::f01659d4ee97efe9714b7fb0ce58fefb https://pubmed.ncbi.nlm.nih.gov/9973108 Zobrazit plný text záznamu Plný text