DEAD-box RNA helicase Dbp4/DDX10 is an enhancer of α-synuclein toxicity and oligomerization

Autor: Blagovesta, Popova, Dan, Wang, Christina, Pätz, Dagmar, Akkermann, Diana F, Lázaro, Dajana, Galka, Miriam, Kolog Gulko, Markus T, Bohnsack, Wiebke, Möbius, Katherine E, Bohnsack, Tiago F, Outeiro, Gerhard H, Braus
Rok vydání: 2020
Předmět:
animal diseases
Yeast and Fungal Models
Toxicology
Pathology and Laboratory Medicine
Biochemistry
DEAD-box RNA Helicases
Antibiotics
Yeasts
Medicine and Health Sciences
Inclusion Bodies
Cytoplasmic Inclusions
Antimicrobials
Eukaryota
Drugs
Neurodegenerative Diseases
Nucleic acids
Protein Transport
Ribosomal RNA
Experimental Organism Systems
Doxycycline
alpha-Synuclein
Hyperexpression Techniques
Saccharomyces Cerevisiae
Protein Binding
Research Article
Amyloid
Cell biology
Cellular structures and organelles
Research and Analysis Methods
Models
Biological
Protein Aggregation
Pathological
Microbiology
Protein Aggregates
Antimalarials
Saccharomyces
Model Organisms
Microbial Control
Gene Expression and Vector Techniques
Humans
Molecular Biology Techniques
Non-coding RNA
Molecular Biology
Pharmacology
Cell Nucleus
Molecular Biology Assays and Analysis Techniques
Toxicity
Organisms
Fungi
Biology and Life Sciences
Nucleolus
Yeast
nervous system diseases
nervous system
Gene Expression Regulation
Animal Studies
RNA
Protein Multimerization
Ribosomes
Zdroj: PLoS Genetics
ISSN: 1553-7404
Popis: Parkinson’s disease is a neurodegenerative disorder associated with misfolding and aggregation of α-synuclein as a hallmark protein. Two yeast strain collections comprising conditional alleles of essential genes were screened for the ability of each allele to reduce or improve yeast growth upon α-synuclein expression. The resulting 98 novel modulators of α-synuclein toxicity clustered in several major categories including transcription, rRNA processing and ribosome biogenesis, RNA metabolism and protein degradation. Furthermore, expression of α-synuclein caused alterations in pre-rRNA transcript levels in yeast and in human cells. We identified the nucleolar DEAD-box helicase Dbp4 as a prominent modulator of α-synuclein toxicity. Downregulation of DBP4 rescued cells from α-synuclein toxicity, whereas overexpression led to a synthetic lethal phenotype. We discovered that α-synuclein interacts with Dbp4 or its human ortholog DDX10, sequesters the protein outside the nucleolus in yeast and in human cells, and stabilizes a fraction of α-synuclein oligomeric species. These findings provide a novel link between nucleolar processes and α-synuclein mediated toxicity with DDX10 emerging as a promising drug target.
Author summary Neurodegenerative Parkinson’s disease affects about 2% of the over 65 years old human population. It is characterized by loss of dopaminergic neurons in midbrain and the presence of Lewy inclusion bodies that are predominantly composed of the α-synuclein protein. Expression of human α-synuclein in yeast cells results in dosage-dependent toxicity monitored as growth reduction and the formation of inclusions similar to mammalian neurons. Systematic analysis of yeast genes, which are essential for growth, revealed that reduced expression of central cellular proteostasis pathways, such as protein synthesis and ubiquitin-dependent protein degradation can enhance or reduce toxic effects of α-synuclein on yeast growth. Expression of α-synuclein affects not only early steps of ribosome biogenesis in yeast but also in human cells. We discovered the nucleolar DEAD-box RNA helicase Dbp4 as a novel strong enhancer of α-synuclein toxicity. The interaction of α-synuclein in yeast with Dbp4 as well as in human cells with its ortholog DDX10 results in sub-cellular exclusion from the nucleolus and promotes the accumulation of toxic oligomeric α-synuclein species. This molecular interaction of α-synuclein with DDX10 and its consequences for human cells provide a novel view in understanding the complexity of Parkinson’s disease.
Databáze: OpenAIRE
Externí odkaz: