Enzyme Kinetics Determined by Single-Injection Isothermal Titration Calorimetry
Autor: | Colette F, Quinn, Lee D, Hansen |
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Rok vydání: | 2019 |
Předmět: | |
Zdroj: | Methods in molecular biology (Clifton, N.J.). 1964 |
ISSN: | 1940-6029 |
Popis: | This chapter describes how to collect Michaelis-Menten kinetic data on an enzyme-catalyzed reaction with the isothermal titration calorimetry (ITC) and the single-injection method. ITC measures the heat rate which is directly proportional to the reaction rate. The enthalpy change (Δ |
Databáze: | OpenAIRE |
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