Enzyme Kinetics Determined by Single-Injection Isothermal Titration Calorimetry

Autor: Colette F, Quinn, Lee D, Hansen
Rok vydání: 2019
Předmět:
Zdroj: Methods in molecular biology (Clifton, N.J.). 1964
ISSN: 1940-6029
Popis: This chapter describes how to collect Michaelis-Menten kinetic data on an enzyme-catalyzed reaction with the isothermal titration calorimetry (ITC) and the single-injection method. ITC measures the heat rate which is directly proportional to the reaction rate. The enthalpy change (Δ
Databáze: OpenAIRE