| Autor: |
P M, Sant'Ana, J E, Oliveira, E R, Lima, C R J, Soares, C N, Peroni, P, Bartolini, Maria Teresa C P, Ribela |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Applied microbiology and biotechnology. 102(3) |
| ISSN: |
1432-0614 |
| Popis: |
A strain of embryonic human kidney cells (HEK293) was transiently co-transfected with the expression vectors coding for the α- and β-subunits of human thyroid-stimulating hormone (hTSH), and, for the first time, a human cell-derived recombinant hTSH was synthesized and extensively characterized. The purification strategy involving two steps provided an overall yield of 55% and a purity level 90%. The purified material (hTSH-HEK) was analyzed and compared to a CHO-derived recombinant preparation (hTSH-CHO) and to a pituitary-derived (hTSH-Pit) preparation. The three preparations showed an equivalent purity ( 95%) with a hTSH-HEK molecular mass 2.1% lower than that of hTSH-CHO and 2.7% higher than that of hTSH-Pit. Remarkable differences were found in the carbohydrate moiety, the lowest sialic acid content and highest fucose content being observed in hTSH-HEK. In vivo biological activity was confirmed for the three preparations, the hTSH-HEK bioactivity being 39 and 16% lower than those of hTSH-CHO and hTSH-Pit, respectively. The hTSH-HEK circulatory half-life (t |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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