Effects of pertussis toxin treatment on human natural killer cell function

Autor:	M M, Whalen, R N, Doshi, A D, Bankhurst
Rok vydání:	1992
Předmět:	Cytotoxicity Immunologic endocrine system Phosphatidylinositols Cell Degranulation Killer Cells Natural Pertussis Toxin Cell Movement Type C Phospholipases Cell Adhesion Cyclic AMP Humans Virulence Factors Bordetella Glucuronidase Signal Transduction Research Article
Zdroj:	Immunology. 76(3)
ISSN:	0019-2805
Popis:	Membranes from highly purified natural killer (NK) cells were ADP ribosylated by treatment with pertussis toxin (PTX). PTX treatment resulted in a single band of 32P incorporation at M(r) 41,600. PTX treatment of NK cells diminished their ability to lyse K562 tumour cells by about 50%. However PTX treatment had no measurable effect on cAMP levels in NK cells. PTX pretreatment also had no effect on the ability of target cells to induce phosphoinositide turnover or on the ability of the NK cells to conjugate with the K562 tumour cells. Movement toward the chemoattractants interleukin-2 (IL-2) and formylmethionylleucylphenylalanine (FMLP) was significantly inhibited indicating that a PTX substrate in NK cells may be involved in the transduction of signals which are involved in cell motility.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::ee7dc946ffd093b36d7089258cff932f https://pubmed.ncbi.nlm.nih.gov/1326477 Zobrazit plný text záznamu Plný text ve formátu PDF