| Autor: |
A M, Diamond, D, Jaffe, J L, Murray, A R, Safa, B L, Samuels, D L, Hatfield |
| Rok vydání: |
1996 |
| Předmět: |
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| Zdroj: |
Biochemistry and molecular biology international. 38(2) |
| ISSN: |
1039-9712 |
| Popis: |
Selenocysteine tRNA[Ser]Sec isoacceptors contain the modified nucleotide i6A immediately 3' to the anticodon. Because synthesis of i6A is expected to be inhibited by lovastatin, the status of tRNA[Ser]Sec isoacceptors was examined in human breast carcinoma cells. As part of the initial characterization of these cells, it was determined that an adriamycin resistant derivative of the MCF-7 cell line exhibited a dramatic increase in the sensitivity to the killing effects of lovastatin relative to the parental MCF-7 cells. When MCF-7Adr cells were incubated with high levels of lovastatin, there was a dramatic perturbation in the distribution of isoacceptors within the selenocysteine tRNA population. Lovastatin may therefore be a useful reagent for both the study of differential killing of drug-resistant tumor cells and selenoprotein biosynthesis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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