| Autor: |
S, Qadeer, M A, Khan, M S, Ansari, B A, Rakha, R, Ejaz, A U, Husna, A, Azam, S, Akhter |
| Rok vydání: |
2021 |
| Zdroj: |
Cryo letters. 40(6) |
| ISSN: |
0143-2044 |
| Popis: |
The quality of cryopreserved buffalo semen is low due to high susceptibility of sperm membranes to cold shock.The present study was designed to investigate the effect of recombinant type-III antifreeze protein from the eel pout Macrozoarces americanus (rAFPIII) on freezability of buffalo semen.Semen was collected from three buffalo bulls for three weeks (replicates). Qualified ejaculates (N=18) were split into four aliquots and diluted in Tris-citric acid extender containing 0.0, 0.1, 1 and 10 µg mLSupplementation of the extender with rAFPIII didn't affect motility and PMI of chilled semen. Post-thaw sperm motility and PMI were higher in extender supplemented with rAFPIII (10µg mLAddition of rAFPIII in extender improved motility and PMI of cryopreserved buffalo semen. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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