Overexpression of p53 in squamous cell carcinoma of the tongue in young patients with no known risk factors is not associated with mutations in exons 5-9

Autor:	M W, Lingen, K W, Chang, S J, McMurray, D B, Solt, M S, Kies, B B, Mittal, G K, Haines, H J, Pelzer
Rok vydání:	2000
Předmět:	Adult Male Adolescent Biopsy Needle Age Factors Exons Genes p53 Prognosis Sensitivity and Specificity Tongue Neoplasms Gene Expression Regulation Neoplastic Risk Factors Mutation Biomarkers Tumor Carcinoma Squamous Cell Humans Female Neoplasm Staging Probability Retrospective Studies
Zdroj:	Headneck. 22(4)
ISSN:	1043-3074
Popis:	This study investigated the status of the p53 tumor suppressor gene in patients less than 40 years of age who had squamous cell carcinoma of the tongue develop with no known risk factors.Histologic sections from 21 patients were prepared from formalin-fixed, paraffin-embedded tissue and were processed for standard immunohistochemistry for detection of the p53 protein. In addition, tumors were evaluated by single-strand conformation polymorphism and by DNA sequencing to identify potential mutations in the conserved exons (5-9) of the p53 gene.Eighty-one percent (17 of 21) of the patients overexpressed p53 by immunohistochemical analysis. However, none of these patients demonstrated mutations in exons 5-9 of the gene.These data suggest that the molecular mechanisms by which the young individuals with no risk factors had altered p53 function in oral squamous cell carcinoma may differ from those of the more typical population of individuals who have this malignancy develop.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::ed43bb4a6dc7cd787fa6165d19315a3e https://pubmed.ncbi.nlm.nih.gov/10862014 Zobrazit plný text záznamu Plný text