| Autor: |
Lin-qing, Yang, Qing-cheng, Liu, Chun-mei, Gong, Gong-hua, Tao, Jian-jun, Liu, Gong-hua, Hu, Hai-yan, Huang, Kun-peng, Wang, Zhi-xiong, Zhuang |
| Rok vydání: |
2011 |
| Předmět: |
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| Zdroj: |
Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases. 29(3) |
| ISSN: |
1001-9391 |
| Popis: |
To construct DNA methyltransferase 1 (DNMT1) low expression 16HBE cell line and observe the variation of cell cycle and global genomic DNA methylation.The method of Lenti-virus induced RNA interference was applied to introduce four different shRNA fragment into 16HBE cells. Flow cytometry and 5-mC immunofluorescence methods were used to observe the cell cycle and global DNA methylation status of DNMT1 low expression 16HBE cells.The DNMT1 protein relative expression level of 16HBE-shDNMT1-4 cell line was down regulated about 44% (P0.05) compared with the control. No obvious differences of cell cycle and global genome DNA methylation status were observed between the 16HBE and 16HBE-shDNMT1.The DNMT1 gene low expression cell is successfully constructed, and there are no obvious changes happened on the cell cycle and global genomic DNA methylation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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