lacZ--Y+ fusions in Escherichia coli. DNA sequencing reveals the eight N-terminal residues of lac permease as non-essential

Autor:	H, Bocklage, B, Müller-Hill
Rok vydání:	1983
Předmět:	DNA Bacterial Base Sequence Monosaccharide Transport Proteins Symporters Escherichia coli Proteins Mutation Escherichia coli Membrane Transport Proteins Amino Acid Sequence Cloning Molecular Codon
Zdroj:	European journal of biochemistry. 137(3)
ISSN:	0014-2956
Popis:	The nucleotide sequence of three lacZ- -Y+ fusions found among spontaneous lacY+ revertants of the lacY- mutant MAB16 in Escherichia coli is reported. MAB16 is a frameshift mutation in codon 6 of the lacY gene. DNA sequence analysis of the fusions shows the first eight N-terminal codons of the lacY gene can be replaced by 3, 39 or 804/805 N-terminal codons of the lacZ gene without impairing lac permease activity qualitatively.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::ec82833d64aa80320734122b77376240 https://pubmed.ncbi.nlm.nih.gov/6363062 Zobrazit plný text záznamu Plný text