| Autor: |
Anna, Kálosi, Martina, Labudová, Adriana, Annušová, Monika, Benkovičová, Michal, Bodík, Jozef, Kollár, Mário, Kotlár, Peter, Kasak, Matej, Jergel, Silvia, Pastoreková, Peter, Siffalovic, Eva, Majkova |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Biomaterials science. 8(7) |
| ISSN: |
2047-4849 |
| Popis: |
We evaluate the application of surfactant-free liquid-phase exfoliated MoS2 nanosheets as a nanoplatform for a cancer detection and treatment system equipped with an antibody-antigen based recognition element. Employing antigen-antibody binding, we increased the probability of the endocytosis of MoS2 nanosheets into CAIX expressing cells by 30%. The nanosheets are functionalized with a specific antibody M75, which forms an antigen-antibody complex with CAIX. The bioconjugation of MoS2 nanosheets involves biocompatible components with low cytotoxicity, verified in the tested cell lines by fluorescence-based cell viability assay. The cellular internalization is quantified by flow cytometry, while the internalization is confirmed by label-free confocal Raman imaging. Raman measurements show increased lysosomal activity in the proximity of the internalized nanoplatforms. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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