[Screening of serum marker proteins in osteosarcoma and preliminary bioinformatic analysis on POLR3F]
| Autor: | Guo-dong, Li, Zheng-dong, Cai, Xiao-jun, Ma, Meng-xiong, Sun, Jian, Li, Wei, Sun, Ying-qi, Hua |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Male STAT3 Transcription Factor Osteosarcoma Binding Sites Adolescent Gene Expression Profiling RNA Polymerase III Bone Neoplasms Blood Proteins Gene Expression Regulation Neoplastic Protein Subunits Young Adult Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Biomarkers Tumor Humans Female RNA Messenger Child Peptides Promoter Regions Genetic Oligonucleotide Array Sequence Analysis Protein Binding |
| Zdroj: | Zhonghua zhong liu za zhi [Chinese journal of oncology]. 33(11) |
| ISSN: | 0253-3766 |
| Popis: | To screen potential serum marker proteins of osteosarcoma, and to make a preliminary bioinformatics analysis of RNA polymerase III polypeptide F (POLR3F).Gene chip and SELDI-TOF MS was used to screen genes differentially expressed in osteosarcoma. The associations of potential biomarkers from SELDI data and microarray analysis were further inferred by link-test to identify biomarkers that could likely be used for diagnosis. MATLAB was used to search transcription factors binding site in the promoter region and miRNAs binding site in 3'-UTR of POLR3F, respectively.653 differentially expressed genes were found in osteosarcoma cells, while six differentially expressed protein peaks with significant statistical significances were detected by SELDI-TOF MS in patient's serum. 13 potential biomarkers for early diagnosis of osteosarcoma were screened by link-test. A conserved STAT3 binding site and a miRNA target site were found in proximal promoter regions and 3'-UTR region of POLR3F, respectively.Link-test is a effective method to identify osteosarcoma biomarkers from both microarray and SELDI-TOF MS database. The results confirmed that POLR3F may be a promising biomarker for early diagnosis and a therapy target of osteosarcoma. |
| Databáze: | OpenAIRE |
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