[Polymerase and immunologic activity of reverse transcriptase of the HIV-1 virus, isolated from Escherichia coli]
| Autor: | Iu P, Shvetsov, A A, Mel'nikov, Z K, Suvorova, T N, Kopylova-Sviridova, V V, Pokrovskiĭ, I I, Fodor |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Acquired Immunodeficiency Syndrome
HIV Antigens Blotting Western RNA-Directed DNA Polymerase DNA-Directed DNA Polymerase Gene Expression Regulation Bacterial Chromatography DEAE-Cellulose Gene Expression Regulation Enzymologic Recombinant Proteins Genes Bacterial Escherichia coli HIV-1 Humans Plasmids |
| Zdroj: | Molekuliarnaia genetika, mikrobiologiia i virusologiia. (3) |
| ISSN: | 0208-0613 |
| Popis: | The recombinant reverse transcriptase of HIV-1 virus has been isolated from Escherichia coli cells transformed by the plasmid pRT40 DNA. The 103 Kd protein produced by these cells is shown to be processed to proteins with lower molecular masses by the reverse transcriptases own protease activity as well as Escherichia coli proteases. The resulting 103-66 Kd proteins possess the polymerase activity while 51 Kd and smaller proteins are lacking the activity. The 66 and 51 Kd reverse transcriptase fragments demonstrate the positive immunological reaction with the human blood serum from the people possessing antibodies to HIV-1 virus. The recombinant reverse transcriptase of HIV-1 produced by Escherichia coli cells is shown to be useful in AIDS diagnosis in humans. |
| Databáze: | OpenAIRE |
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