| Autor: |
P H, Culbreth, I W, Duncan, C A, Burtis |
| Rok vydání: |
1977 |
| Předmět: |
|
| Zdroj: |
Clinical chemistry. 23(12) |
| ISSN: |
0009-9147 |
| Popis: |
We used paired-ion high-performance liquid chromatography to determine the 4-nitrophenol content of 4-nitrophenyl phosphate, a substrate for alkaline phosphatase analysis. This was done on a reversed-phase column with a mobile phase of methanol/water, 45/55 by vol, containing 3 ml of tetrabutylammonium phosphate reagent per 200 ml of solvent. At a flow rate of 1 ml/min, 4-nitrophenol was eluted at 9 min and monitored at 404 nm; 4-nitrophenyl phosphate was eluted at 5 min and could be monitored at 311 nm. Samples of 4-nitrophenyl phosphate obtained from several sources contained 0.3 to 7.8 mole of 4-nitrophenol per mole of 4-nitrophenyl phosphate. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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