| Popis: |
To screen for the most stable reference genes(RGs) in various tissues of rats fed at different dietary concentrations of selenium(Se).Twenty-four weaning male SD rats were fed Se deficient diet for 5 weeks, and then randomly divided into 4 groups for0. 01, 0. 25, 3 and 5 mg Se/kg diet feeding, respectively. After 4 weeks, animals were sacrificed for sample collection of liver, testis, muscle and fat tissue. Twelve candidate RGs of Actb, Atp5f1, B2m, Gapdh, Gusb, Hprt, Pgk1, Ppia, Rplp2, Rps18, Tbp and Ywhaz were tested for their quantitative cycle numbers of mRNA abundances with the quantitative PCR method. The stabilities of the candidate RGs were evaluated by the arithmetic packages of geNorm, NormFinder, BestKeeper, Delta CT and RefFinder.The top 4 most stable RGs were PpiaAtp5f1Rplp2Hprt in liver; YwhazAtp5f1Rplp2Ppia in testis; TbpPpiaB2mRps18 in muscle; HprtTbpAtp5f1Pgk1 in fat tissue; and Rps18HprtRplp2Atp5f1 when all the 4 tissues combined for analysis.To analyze the expressions of the target genes in rats fed different concentrations of dietary Se, the best RGs should be selected depending on the tissue types. |
